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High-performance liquid chromatographic determinations of disaccharides resulting from enzymatic degradation of isomeric chondroitin sulfates

✍ Scribed by George Jia-Long Lee; Tieckelmann Howard

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
416 KB
Volume
94
Category
Article
ISSN
0003-2697

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✦ Synopsis

The use of Whatman Partisil-10 PAC and Altex LiChrosorb NH, bonded phase columns for the high-performance liquid chromatographic separation of the disaccharides obtained from digestion of isomeric chondroitin sulfates with chondroitinases has been investigated. The substituted unsaturated disaccharides in the enzymatic degradation mixtures undergo rapid isocratic separations on both bonded stationary phases. A complete separation can be established within 10 min. The development of these procedures has expedited enzymatic studies of isomeric chondroitin sulfates.

The rate of depolymerization of chondroitin sulfates by the action of chondroitinases based on quantitative high-performance liquid chromatographic separations and detection -_ of the disaccharide products was studied. -

πŸ“œ SIMILAR VOLUMES

Enzymatic analysis with chondrosulfatase
Enzymatic analysis with chondrosulfatases of constituent disaccharides of sulfated chondroitin sulfate and dermatan sulfate isomers by high-performance liquid chromatography
✍ Katsumi Murata; Y. Yokoyama πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 689 KB

Various under-sulfated, monosulfated, and over-sulfated chondroitin sulfate and dermatan sulfate isomers were analyzed in terms of disaccharide units before or after desulfation with chondrosulfatases in addition to digestion with chondroitinases. The unsaturated disaccharides were separable by a hi