High-performance liquid chromatographic assay of biphenyl metabolism by hepatocytes cultured in an embryo/hepatocyte co-culture medium
β Scribed by M.Leonard Mole; Linda Sanders; Linda A. Oglesby
- Book ID
- 107714609
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 937 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0003-2697
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An isocratic reverse-phase high-performance liquid chromatography method for the separation and quantitation of total pyridine dinucleotides in hepatocyte cultures is described. Cells are extracted with cold 3 M perchloric acid or 0.5 N sodium hydroxide containing 50% (v/v) ethanol and 35% cesium ch
A high-pressure liquid chromatography method was developed to separate 2-aminofluorene and several ring-hydroxylated metabolites. The acetylated derivatives of these compounds and N-hydroxy-2-acetylaminofluorene were also separated simultaneously. HPLC analyses were performed using a DuPont Zorbax C