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High-performance liquid chromatographic analysis of 2-aminofluorene and its metabolites in monolayer cultures of rat hepatocytes

✍ Scribed by Mary Jo Miller; Charlene A. McQueen

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
521 KB
Volume
153
Category
Article
ISSN
0003-2697

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✦ Synopsis

A high-pressure liquid chromatography method was developed to separate 2-aminofluorene and several ring-hydroxylated metabolites. The acetylated derivatives of these compounds and N-hydroxy-2-acetylaminofluorene were also separated simultaneously. HPLC analyses were performed using a DuPont Zorbax C-8 HPLC column and a solvent mixture of 0.02 M acetic acid and isopropanol. Isopropanol concentrations were increased from 27 to 100% over 45 min using a concave gradient system. Desferal mesylate was added to the aqueous component to improve the resolution of several hydroxylated arylamine metabolites. The method was used to quantitate the metabolites of 2-aminofluorene in monolayer cultures of rat hepatocytes.

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