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High-performance liquid chromatography assay of bleomycin in human plasma and rat hepatocytes in culture

✍ Scribed by Rachid Mahdadi; Abdelraouf Kenani; Nicole Pommery; Jean Pommery; Jean Pierre Hénichart; Michel Lhermitte

Publisher
Springer
Year
1991
Tongue
English
Weight
532 KB
Volume
28
Category
Article
ISSN
0344-5704

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An isocratic reverse-phase high-performance liquid chromatography method for the separation and quantitation of total pyridine dinucleotides in hepatocyte cultures is described. Cells are extracted with cold 3 M perchloric acid or 0.5 N sodium hydroxide containing 50% (v/v) ethanol and 35% cesium ch