High-performance liquid chromatographic assay for nanogram determination of chlorpromazine and its comparison with a radioimmunoassay

✍ Scribed by K. K. Midha; J. K. Cooper; I. J. McGilveray; A. G. Butterfield; J. W. Hubbard

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 512 KB
• Volume: 70
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600700920

✦ Synopsis

A specific and sensitive high-performance liquid chromatographic (HPLC) method for the quantitative determination of plasma chlorpromazine concentrations is described. The procedure is capable of determining 1 ng of chlorpromazine/ml and is adequate for following plasma concentration-time profiles after 7-mg single intravenous doses. After a simple organic extraction of the drug and an internal standard (mesoridazine) from plasma, the organic layer was transferred to a vial and evaporated to dryness at 55 degrees under nitrogen. The residue was dissolved in 200 microliters of HPLC grade acetonitrile. Aliquots (70-100 microliters) were chromatographed, and the drug was quantitated in the range of 1-15 ng/ml of plasma using a fixed-wavelength UV detector. Plasma concentrations determined by the method were compared with those obtained by a previously reported radioimmunoassay specific for chlorpromazine and N-desmethylchlorpromazine. The two methods agreed favorably with a correlation coefficient of 0.993 and a slope of 0.994.