High-Performance Liquid Chromatographic Analysis of Cyclosporine A in Human Skin

✍ Scribed by Aleš Husek; Ernst Eibler; Wolfgag Wiegrebe

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 340 KB
• Volume: 326
• Category: Article
• ISSN: 0365-6233
• DOI: 10.1002/ardp.19933260610

✦ Synopsis

A three-dimensional high-performance liquid chromatography (HPLC) method is described for the determination of cyclosporine A in human skin. The method allows to determine at least 3 ng cyclosporine A in 10 mg skin (total sample amount needed) and is suitable for cyclosporine A level monitoring in the skin of psoriatic patients.

HPLC-Bestimmung von Cyclosporin A in menschlicher Haut

Eine dreidimensionale HPLC-Methode zur Bestimmung von < 3 ng Cyclosporin A in I0 mg menschlicher Haut wird beschrieben. Dieses Verfahren eignet sich zur Cyclosporin A Bestimmung in der Haut von Psoriatikern.

Cyclosporine A (CyA) is highly effective in the systemic treatment of psoriasis, The mechanism of CyA functioning in skin is not yet fully understood, but it is known that topical application has no clinical effect. However, by oral or intravenous application dose-related side-effects, particularly nephrotoxicity, appeared').