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High-performance liquid chromatographic analysis of ammonium in human urine

✍ Scribed by Munehiko Mizobuchi; Kikuo Tamase; Yoshimi Kitada; Michiko Sasaki; Kaoru Tanigawa

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
344 KB
Volume
137
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for the quantitative determination ofammonium in human urine by high-performance liquid chromatography (HPLC) is described. After making fluorescent substances with fluorescamine. they were separated and quantified by their fluorometric intensity. The intensity (as measured by peak height) was linear between 0.5 and 5.0 pg, and coefficients of variation for elution time and peak height on replicate analysis of the standard were 0. I5 and 4.2%. respectively. Recoveries of added ammonium were 96.5 and 97.3'%, respectively, on 2.0 and 3.0 Fg by this method. Detection limit of this method was 0.2 pg. There was good agreement between the proposed HPLC method (X) and ion chromatographic method ( Y). giving the relationships as Y = 0.956X + 0.012. r = 0.991.

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