High-performance affinity chromatography of proteins using non-porous silica beads

✍ Scribed by Wen-Chien Lee; Chien-Yi Chuang; Ching-Lun Chen; Chen-Yaw Chiu

• Book ID: 104645700
• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 273 KB
• Volume: 8
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00154319

✦ Synopsis

Non-porous silicas with an average diameter of 1.4 pm were prepared for rapid analytical and micropreparative high-performance affinity chromatography of proteins. The non-porous silica was silanized with y -aminopropyltriethoxy silane to introduce terminal amino groups on which protein A was immobilized via the activation of glutaraldehyde. When the column packed with the resultant protein A-silica, the retained component of the sample could be eluted by a stepwise change of pH from 7 to 3. A linear relationships was observed between the peak height (or peak area) and the IgG content in the sample. The data indicated that the sample with IgG content up to ca. 300 pg could be chromatographically analysed.