Non-porous silicas with an average diameter of 1.4 pm were prepared for rapid analytical and micropreparative high-performance affinity chromatography of proteins. The non-porous silica was silanized with y -aminopropyltriethoxy silane to introduce terminal amino groups on which protein A was immobi
Analysis of lidocaine interactions with serum proteins using high-performance affinity chromatography
β Scribed by Sony Soman; Michelle J. Yoo; Yoon Jeong Jang; David S. Hage
- Book ID
- 108161129
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 263 KB
- Volume
- 878
- Category
- Article
- ISSN
- 1873-376X
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## Abstract The peak decay method is an affinity chromatographic technique that has been used to examine the dissociation of solutes from immobilized ligands in the presence of excess displacing agent. However, it can be difficult to find a displacing agent that does not interfere with detection of