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High-performance liquid affinity chromatography of liver plasma membrane proteins

✍ Scribed by Djuro Josić; Werner Hofmann; Ralf Habermann; Andreas Becker; Werner Reutter

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
894 KB
Volume
397
Category
Article
ISSN
1873-3778

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✦ Synopsis

Plasma membrane proteins from liver were analysed by concanavalin A affinity and immunoaffinity high-performance liquid chromatography. In the method, four peptide bands with apparent molecular weights of 140,000, 120,000, 80,000 and 60,000 could be isolated. In the second method, with two immobilized monoclonal antibodies, two corresponding antigens--the membrane proteins dipeptidyl-peptidase IV and GP 110--could be highly purified from plasma membrane extract with good yield in only one step.

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