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High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells

✍ Scribed by Amegadzie, Bernard Y.; Jiampetti, Deborah; Craig, Robert J.; Appelbaum, Edward; Shatzman, Allan R.; Mayer, Ruth J.; DiLella, Anthony G.


Book ID
123326851
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
323 KB
Volume
128
Category
Article
ISSN
0378-1119

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A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF-B/Rel family of proteins. Infection of Spodoptera frugiderda insect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting u