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PRODUCTION OF BIOLOGICALLY ACTIVE HUMAN RELA (p65) IN BACULOVIRUS-INFECTED INSECT CELLS

✍ Scribed by MARIE CHABOT-FLETCHER; MAUREEN L. HO; JOHN J. BRETON; CHARLES R. HANNING; BERNARD Y. AMEGADZIE

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 292 KB
Volume: 20
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.1996.0100

No coin nor oath required. For personal study only.

✦ Synopsis

A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF-B/Rel family of proteins. Infection of Spodoptera frugiderda insect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting using RelA-specific antisera and by electrophoretic mobility shift assays. The recombinant protein bound specifically to an oligonucleotide containing the NF-B consensus motif but not to that containing the unrelated Oct-1 consensus motif. Thus insect cell-derived RelA possess properties similar to the native protein and may be used in physical, biochemical, and pharmacological studies.

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