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Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells

✍ Scribed by Pedro E. Cruz; Cristina C. Peixoto; José L. Moreira; Manuel J. T. Carrondo

Publisher
Wiley (John Wiley & Sons)
Year
1998
Tongue
English
Weight
299 KB
Volume
72
Category
Article
ISSN
0268-2575

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✦ Synopsis

In this work Sf-9 cells previously adapted to SF900II and EXCELL 401 serum-free media (SFM) were grown and infected at di †erent agitation rates in order to study the e †ects of power input upon cell growth, infection and production of Pr55gag particles in both SFM. Maximum cell concentration increased from 3 ] 106 to 6 ] 106 cells cm~3 and 6É5 ] 106 cells cm~3 when the agitation increased from 80 to 250 rpm or sparged aeration (0É01 vvm at 170 rpm) is used, clearly indicating that cell growth is limited by gas transfer. The speciÐc productivity increased 3É5-fold when the agitation rate was increased from 80 to 170 rpm, indicating that in SFM cell infection is also limited by gas transfer. The highest product concentration was obtained in SF900II at 120 h post-infection (hpi). The product quality analysis showed that SF900II is the best medium for production of Pr55gag particles and that a careful optimisation of the harvest time is required. The maximum product titre was obtained at 120 hpi, 48 h after the achievement of the highest quality. 1998

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