High-Level Expression of Biologically Active Human Prolactin from Recombinant Baculovirus in Insect Cells
β Scribed by Tapas Das; Paul W. Johns; Vincent Goffin; Paul Kelly; Bruce Kelder; John Kopchick; Kristi Buxton; Pradip Mukerji
- Book ID
- 115645708
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 138 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1046-5928
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A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF-B/Rel family of proteins. Infection of Spodoptera frugiderda insect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting u
In order to develop an efficient process for large-scale production of recombinant protein, various factors were studied which affect the productivity of Sf-9 (Spodoptera frugiperda) insect cells when using the baculovirus expression system. It was shown that upon infection with the Bac-BRV6L recomb