High-Level Expression of Escherichia coli and Bacillus subtilis Thymidylate Synthases
β Scribed by Li-Ming Changchien; Araik Garibian; Verna Frasca; Angelo Lobo; Gladys F. Maley; Frank Maley
- Book ID
- 115645663
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 92 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1046-5928
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We have cloned a 14 kb DNA segment containing the coding sequence (polC) for DNA polymerase III (PolIII) from the Bacillus subtilis chromosome. The plasmid carrying the sequence, pRO10, directs the synthesis of the 160 kDa PolIII protein and three additional polypeptides in Escherichia coli maxicell
Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferas