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Metabolic flux analysis of Escherichia coli expressing the Bacillus subtilis acetolactate synthase in batch and continuous cultures

✍ Scribed by Aristos A. Aristidou; Ka-Yiu San; George N. Bennett

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 731 KB
Volume: 63
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19990620)63:6<737::aid-bit12>3.0.co;2-9

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## Abstract A novel metabolic engineering technique involving the redirection ofcellular carbon fluxes was employed to reduce acetate production in an __Escherichia coli__ culture. Metabolic engineering was achieved by cloning __E. coli__ the gene for the __Bacillus subtilis__ acetolactate synthase