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High-level expression of Bacillus stearothermophilus 6-phosphofructo-l-kinase in Escherichia coli

✍ Scribed by French, Brent A.; Valdez, Benigno C.; Younathan, Ezzat S.; Chang, Simon H.


Book ID
122024483
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
586 KB
Volume
59
Category
Article
ISSN
0378-1119

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Cloning and expression of a thermophilic
✍ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII