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High Level Expression of Streptokinase in Escherichia Coli

✍ Scribed by Estrada, M. P. ;Hernandez, L. ;Pérez, A. ;Rodriguez, P. ;Serrano, R. ;Rubiera, R. ;Pedraza, A. ;Padrón, G. ;Antuch, W. ;Fuente, J. de la ;Herrera, L.

Book ID
121678082
Publisher
Nature Publishing Group
Year
1992
Tongue
English
Weight
780 KB
Volume
10
Category
Article
ISSN
1087-0156

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High-level expression and secretion of streptokinase inEscherichia coli
✍ Jae Hyeong Ko; Do Keun Park; Il Chul Kim; Si Hyoung Lee; Si Myung Byun 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 364 KB

The high-level expression plasmid for streptokinase, pSK100, has been constructed. It contains a tac promoter, an ompA signal sequence, a streptokinase structural gene(skc) and a rrnBT1T2 transcription terminator. E. coli JM109 carrying pSK100 produced about 5,000IU of streptokinase per 1 ml of LB-a