A new strategy for the characterization of Coomassie Brilliant Blue stained SDS-PAGE separated proteins by UV-MALDI-MS is reported. The proteins are extracted directly from the polyacrylamide gel by treatment with an organic solvent mixture consisting of formic acid, acetonitrile, isopropanol and wa
High-efficiency protein extraction from polyacrylamide gels for molecular mass measurement by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry
β Scribed by Ya Jin; Takashi Manabe
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 774 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
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