✦ LIBER ✦

Sample preparation for high throughput accurate mass analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Scribed by Derek A. Lake; Murray V. Johnson; Charles N. McEwen; Barbara S. Larsen

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 79 KB
Volume: 14
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(20000615)14:11<1008::aid-rcm979>3.0.co;2-t

No coin nor oath required. For personal study only.

✦ Synopsis

An automated sample preparation for high throughput accurate mass determinations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been developed. Sample preparation was performed with an automated workstation and automated mass analyses were performed with a commercial MALDI-TOF mass spectrometer. The method was tested with a 41-sample library. MALDI-TOFMS was found to give the needed sensitivity, accurate mass measurement, and soft ionization necessary for structure confirmation, even of mixtures. A mass accuracy of 5 ppm or less was obtained in over 80% of known compound measurements. A mass accuracy better than 10 ppm was obtained for all measurements of known compounds. Analyses of parallel synthesis products resulted in 77% of the measurements with a mass accuracy of 5 ppm or better.

📜 SIMILAR VOLUMES

Electrospray sample preparation for impr

Electrospray sample preparation for improved quantitation in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Russell R. Hensel; Richard C. King; Kevin G. Owens 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB 👁 1 views

The goal of this work is the development of a rapid and objective matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) method for the quantitation of peptides and proteins in human plasma suitable for use in the Good Laboratory Practices (GLP) environment, where

Quantitative analysis of antibiotics by

Quantitative analysis of antibiotics by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Yong-Chien Ling; Lihnian Lin; Yi-Ting Chen 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 242 KB 👁 1 views

Comparative studies of the matrix-assisted laser desorption/ionization (MALDI) of 30 antibiotics were made using a-cyano-4-hydroxycinnamic acid (HCCA), 2,5-dihydroxybenzoic acid (DHB), 5,10,15,20tetrakis(4-hydroxyphenyl)-21H,23H-porphyrin and meso-tetra(N-methyl-4-pyridyl)porphyrin matrices. Most an

Sample Preparation Optimization for the

Sample Preparation Optimization for the Analysis of Gliadins in Food by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

✍ Camafeita, Emilio; Alfonso, Patricia; Acevedo, Boris; Méndez, Enrique 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 318 KB 👁 1 views

Analysis by matrix-assisted laser desorption/ionization time-of-Ñight mass spectrometry (MALDI/TOF-MS) of wheat gliadin extracts from distinct types of wheat varieties displays a similar characteristic gliadin mass pattern within the 30-40 kDa range. Direct observation of this pattern in food sample

Simplified sample preparation for the an

Simplified sample preparation for the analysis of oligonucleotides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

✍ G. John Langley; Julie M Herniman; Nichola L Davies; Tom Brown 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 106 KB 👁 1 views

Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) for the analyses of oligonucleotides has generally been carried out using negative ionisation conditions, usually following ammonium ion-exchange chromatography and the addition of ammonium buffers to the MALD

Analysis of solid-supported oligonucleot

Analysis of solid-supported oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Albert Meyer; Nicolas Spinelli; Jean-Louis Imbach; Jean-Jacques Vasseur 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 134 KB 👁 1 views

This study presents matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) as a powerful tool to analyze and characterize oligonucleotides covalently linked to a solid support during their synthesis. The analysis of the fragment ions generated either in negative o

Accurate peptide sequencing by post-sour

Accurate peptide sequencing by post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Wenjun Mo; Toshifumi Takao; Yasutsugu Shimonishi 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB 👁 1 views

Partial 18 O-labeling of peptides has been applied to post-source decay experiments in a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The ions which originate from the carboxyl terminus of a peptide partially retain 18 O atoms which have readily been incorporated int