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HFE gene mutations analysis in Basque hereditary haemochromatosis patients and controls

✍ Scribed by Cuadrado, Emilio; de Juan, MaDolores; Reta, Ana; Castiella, Agustin; Pozueta, Jasone; Prada, Alvaro


Book ID
110025206
Publisher
Nature Publishing Group
Year
2001
Tongue
English
Weight
87 KB
Volume
9
Category
Article
ISSN
1018-4813

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## Detection conditions sequence of primers: F: ACATGGTTAAGGCCTGTTGC R: GCCACATCTGGCTTGAAATT PCR conditions: 3 min at 94oC; 30 cycles of 1 min 94oC, 1 min at 62oC and 1 min at 72oC; 10 min at 72oC electrophoresis: SSCP -10% native polyacrylamide gel (37.5:1 acrylamide: bis-acrylamide), 0.5x TBE,