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Hexose transport in plasma membrane vesicles prepared from L6 rat myoblasts

✍ Scribed by O. Mesmer; M.O. Cheung; T. D'Amore; T.C.Y. Lo


Book ID
117057146
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
328 KB
Volume
141
Category
Article
ISSN
0006-291X

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Isolation and characterization of hexose
✍ Tony D'Amore; Vincent Duronio; Matthias O. Cheung; Theodore C. Y. Lo πŸ“‚ Article πŸ“… 1986 πŸ› John Wiley and Sons 🌐 English βš– 878 KB

A method tor the selection and isolation ot hexose transport mutants in unditterentiated rat myoblast L6 cells is reported; 2-deoxy-D-glucose (2-DOG)and 2-deoxy-2-fluoro-D-glucose (2FG)-resistant mutants were selected after mutagenization of L6 cells with ethyl methanesulfonate. Of these, D18 and D2

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## N6A 5C1 OMG was transported exclusively by the sulfhydryl reagent insensitive low affinity system (Christopher et al., 1976). In this manuscript, we report the effects of sulfhydryl reagents on hexose uptake in undifferentiated L6 rat myoblasts. As in the case with chick embryo fibroblasts, the