𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and characterization of hexose transport mutants in L6 rat myoblasts

✍ Scribed by Tony D'Amore; Vincent Duronio; Matthias O. Cheung; Theodore C. Y. Lo

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
878 KB
Volume
126
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

A method tor the selection and isolation ot hexose transport mutants in unditterentiated rat myoblast L6 cells is reported; 2-deoxy-D-glucose (2-DOG)and 2-deoxy-2-fluoro-D-glucose (2FG)-resistant mutants were selected after mutagenization of L6 cells with ethyl methanesulfonate. Of these, D18 and D23 (selected with 0.1 mM 2-DOC) and F72 and F76 (selected with 0.1 m M 2FC) exhibited the lowest hexose transport activity. Uptake of 0.06 mM 2-DOG, 2FG, or 3-0-methyl-D-glucose (3-OMG) by mutants grown in tructose medium supplemented with 0.05 mM 2FG was about fourto five-fold lower than the parental L6 cells. These mutants contain normal levels ot ATP and glycolytic enzyme activities. They also exhibit normal transport activities for a-aminoisobutyric acid and fructose. Furthermore, hexose transport was observed to be decreased in plasma membrane vesicles prepared from these mutants. Kinetic analysis ot 2-DOG and 3-OMG transport in mutant F72 demonstrated that the V , , , , tor 2-DOG uptake was signiticantly reduced, whereas the V , , ,

πŸ“œ SIMILAR VOLUMES

Hexose transport in L6 rat myoblasts. II
Hexose transport in L6 rat myoblasts. II. The effects of sulfhydryl reagents
✍ Tony D'amore; Theodore C. Y. Lo πŸ“‚ Article πŸ“… 1986 πŸ› John Wiley and Sons 🌐 English βš– 757 KB

## N6A 5C1 OMG was transported exclusively by the sulfhydryl reagent insensitive low affinity system (Christopher et al., 1976). In this manuscript, we report the effects of sulfhydryl reagents on hexose uptake in undifferentiated L6 rat myoblasts. As in the case with chick embryo fibroblasts, the

Isolation and characterization ofKluyver
Isolation and characterization ofKluyveromyces marxianus mutants deficient in malate transport
✍ QueirΓ³s, O.; Casal, M.; Althoff, S.; Moradas-Ferreira, P.; LeΓ£o, C. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 136 KB

In malic acid-grown cells of the strains ATCC 10022 and KMS3 of Kluyveromyces marxianus the transport of malic acid occurred by a malate-proton symport, which accepted -malic, -malic, succinic and fumaric acids, but not tartaric, malonic or maleic acids. The system was inducible and subjected to g

Characterization of L-DOPA transport in
Characterization of L-DOPA transport in cultured rat and mouse astrocytes
✍ M.J. Tsai; E.H.Y. Lee πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 583 KB

The present work studied the transport of L-DOPA in cultured rat and mouse astrocytes. Results indicated that the uptake of L-[14C]DOPA in both rat and mouse astrocytes was Na+ independent and temperature sensitive. It was mediated by a carrier-mediated mechanism with K, values of 36 and 60.3 pM, an

Characterization of guanine and guanosin
Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons
✍ Kazuki Nagasawa; Fumio Kawasaki; Akiko Tanaka; Katsuhito Nagai; Sadaki Fujimoto πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 390 KB

## Abstract In this study, we examined the transport mechanisms for guanine and guanosine in rat neurons and astrocytes, and compared their characteristics. In the both types of cell, the uptake of [^3^H]guanine and [^3^H]guanosine was time‐, temperature‐, and concentration‐dependent, and Na^+^‐ind

Isolation and characterization of metabo
Isolation and characterization of metabolites of centpropazine in rat liver, intestine, and red blood cell homogenates
✍ Bhattaram V. Atul; Shio K. Singh; K. P. Madhusudanan; Jyoti K. Paliwal; Ram C. G πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 145 KB

The potential sites for metabolism of centpropazine (CPZ) (an antidepressant) were evaluated in male Sprague-Dawley rats. The isolation and identification of the major metabolites formed in the presence of rat liver S9 fraction, intestine, and red blood cells under aerobic conditions were performed