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Heterologous expression in Pichia pastoris and single-step purification of a cysteine proteinase from northern shrimp

✍ Scribed by Hitoshi Aoki; Md Nazmul Ahsan; Shugo Watabe


Book ID
117702819
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
355 KB
Volume
31
Category
Article
ISSN
1046-5928

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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RT‐PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5β€² alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t