Heterogeneity of HLA genetic factors in IDDM susceptibility

✍ Scribed by M. Martell; A. Marcadet; A. Moine; C. Boitard; I. Deschamps; J. Dausset; J. F. Bach; D. Cohen

• Publisher: Springer-Verlag
• Year: 1990
• Tongue: English
• Weight: 781 KB
• Volume: 31
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00204893

✦ Synopsis

The association of certain HLA-D alleles with insulin-dependent diabetes mellitus (IDDM) is well known. One hundred and sixty-one non-related diabetic individuals and 142 non-related healthy controls were typed for the HLA DR-DQw-Dw association, using a restriction fragment length polymorphism (RFLP) typing method that combines three probe/enzyme systems: DRB/Taq I, DQB/Taq I, and DQB/Bam HI. Comparison of frequencies in both diabetics and controls confirms previous results in terms of HLA class II and IDDM association. Moreover, we have found that DR3/3 heterozygous individuals are more susceptible to IDDM when they are also Dw25 (associated with B18) than when they are Dw24 (associated with B8). Using oligonucleotide dot-blot hybridizations we analyzed the HLA-DQB1 sequence of DR3,Dw24 and DR3,Dw25 homozygous individuals, and we found no difference at position 57 between these two DR3-carrying haplotypes. This observation points to the heterogeneity of HLA genetic factors in IDDM susceptibility.