Hereditary monocyte esterase deficiency
β Scribed by Geraldine M. Markey; H. D. Alexander; Robyn Mcconnell; Anne Kyle; T. C. M. Morris; J. H. Robertson
- Book ID
- 114710871
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 858 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0007-1048
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## Abstract Monocytes from five unrelated children (four boys and a girl) with chronic granulomatous disease were studied for their ability to reduce nitroblue tetrazolium dye after stimulation with zymosan, and for their Ξ±βnaphtyl butyrate esterase activity. As expected, monocytes ingested zymosan
Esterase from monocytes promotes the hydrolysis of 2-methyl-1-propenylbenzoate (MPB) yielding 2-methyl-1-propenol, which is oxidized by horseradish peroxidase/H 2 O 2 producing triplet acetone. The chemiluminescence of this reaction can be enhanced by the addition of 9,10-dibromoanthracene-2-sulphon