Chemiluminescent determination of esterases in monocytes

✍ Scribed by Fonseca, Luiz Marcos da ;Yavo, Boni ;Catalani, Luiz Henrique ;Falcão, Roberto P. ;Brunetti, Iguatemy Lourenço ;Campa, Ana

• Book ID: 101289810
• Publisher: John Wiley and Sons
• Year: 1998
• Weight: 187 KB
• Volume: 13
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/(sici)1099-1271(199807/08)13:4<195::aid-bio497>3.0.co;2-p

✦ Synopsis

Esterase from monocytes promotes the hydrolysis of 2-methyl-1-propenylbenzoate (MPB) yielding 2-methyl-1-propenol, which is oxidized by horseradish peroxidase/H 2 O 2 producing triplet acetone. The chemiluminescence of this reaction can be enhanced by the addition of 9,10-dibromoanthracene-2-sulphonate. The non-speci®c esterase present in monocytes is responsible for MPB hydrolysis, since (a) the chemiluminescence of the reaction was inhibited by ¯uoride, and (b) cells that do not contain a signi®cant amount of non-speci®c esterases, e.g. lymphocytes and neutrophils, did not trigger light emission. The analytical application of this reaction is considered.