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Henry Thomson Lynch, un itinéraire exemplaire de la génétique clinique

✍ Scribed by C. Matuchansky

Publisher
Springer-Verlag
Year
2007
Tongue
French
Weight
717 KB
Volume
37
Category
Article
ISSN
0240-642X

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The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. su