Analyse génétique de sacB, gène de struc
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Steinmetz, Michel ;Le Coq, Dominique ;Djemia, Hajer Ben ;Gay, Philippe
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Article
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1983
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Springer
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English
⚖ 936 KB
The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. su