Heat- and alkali-induced deamination of 5-methylcytosine and cytosine residues in DNA
โ Scribed by Richard Y.-H. Wang; Kenneth C. Kuo; Charles W. Gehrke; Lan-Hsiang Huang; Melanie Ehrlich
- Book ID
- 119119007
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 504 KB
- Volume
- 697
- Category
- Article
- ISSN
- 0167-4781
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
To analyse the biologica1 role of 5-methylation of cytosine residues in DNA requires precise and efficient methods for detecting individua1 5-methylcytosines (5-MeCs) in genomic DNA. The methods developed over the past decade rely on either differential enzymatic or chemical cleavage of DNA, or more
A method combining gas chromatography and mass spectrometry (GC-MS) with multiple specific ion monitoring has been developed for the detection of S-methylcytosine and the quantitation of the ratio of methylcytosine to cytosine in DNA. The trimethylsilyl derivatives of cytosine and S-methylcytosine o