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Genes and genomes: Sequencing 5-methylcytosine residues in genomic DNA

✍ Scribed by Geoffrey Grigg; Susan Clark

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 847 KB
Volume: 16
Category: Article
ISSN: 0265-9247
DOI: 10.1002/bies.950160612

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✦ Synopsis

To analyse the biologica1 role of 5-methylation of cytosine residues in DNA requires precise and efficient methods for detecting individua1 5-methylcytosines (5-MeCs) in genomic DNA. The methods developed over the past decade rely on either differential enzymatic or chemical cleavage of DNA, or more recently on differential sensitivity to chemical conversion of one base to another. The most commonly used methods for studying the methylation profile of DNA, including the bisulphite base-conversion method, are reviewed.

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