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Site-Specific Discrimination of Cytosine and 5-Methylcytosine in Duplex DNA by Peptide Nucleic Acids

โœ Scribed by Okamoto, Akimitsu; Tanabe, Kazuhito; Saito, Isao


Book ID
126046780
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
75 KB
Volume
124
Category
Article
ISSN
0002-7863

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Sensitive detection of 5-methylcytosine
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A method combining gas chromatography and mass spectrometry (GC-MS) with multiple specific ion monitoring has been developed for the detection of S-methylcytosine and the quantitation of the ratio of methylcytosine to cytosine in DNA. The trimethylsilyl derivatives of cytosine and S-methylcytosine o