𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Growth of insect cells in recycled medium and the use of various serum supplements

✍ Scribed by P. L. Roberts

Publisher
Springer Netherlands
Year
1984
Tongue
English
Weight
368 KB
Volume
6
Category
Article
ISSN
0141-5492

No coin nor oath required. For personal study only.

✦ Synopsis

Medium that had supported the growth of insect cells was depleted in components of both the basal medium and foetal calf serum. In addition, a non-dialysable growth-inhibiting factor(s) appeared to exist. The pH had risen to a sub-optimal level and simply readjusting this to pH 6.6 partially restored its capacity to support cell growth. The most promising alternative serum supplement tested was new-born calf serum.

πŸ“œ SIMILAR VOLUMES

Growth of kidney epithelial cells in hor
Growth of kidney epithelial cells in hormone-supplemented, serum-free medium
✍ Taub, Mary ;Sato, Gordon H. πŸ“‚ Article πŸ“… 1979 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 747 KB

Madin Darby canine kidney cells can grow in synthetic medium supplemented with 5 factors ~ insulin. transferrin, prostaglandin E , , hydrocortisone and triiodothyronine ~-as a seruni substitute. These 5 factors permit growth for one month in the absence of serum, and a growth rate equivalent t o tha

Effect of lipids on insect cell growth a
Effect of lipids on insect cell growth and expression of recombinant proteins in serum-free medium
✍ Rebecca S. Gilbert; Yuichi Nagano; Tadafumi Yokota; Sun Fon Hwan; Tom Fletcher; πŸ“‚ Article πŸ“… 1996 πŸ› Springer 🌐 English βš– 342 KB

The lipid emulsion components of a serum-free insect cell medium were varied and evaluated for effects on cell growth and recombinant protein expression. The growth of High-Five(TM) cells was significantly affected by polyol Pluronic F-68 and Tween-80, but not by lipids. Pluronic was essential for c

Growth of mouse plasmacytoma cells in se
Growth of mouse plasmacytoma cells in serum-free, hormone-supplemented medium: Procedure for the determination of hormone and growth factor requirements for cell growth
✍ Hiroki Murakami; Hideo Masui; Gordon Sato; William C. Raschke πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 676 KB

The procedure of analyzing hormone and growth factor requirements for the growth of MPC-I I cells and of developing a serum-free medium for this cell line has been described. In this medium, MPC-I I cells grow as fast as in serum-supplemented medium, up to 50 generations. MPC-I I cells grown in ser

Egg yolk lipoprotein, a new supplement f
Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium
✍ Hiroki Murakami; Yuichi Okazaki; Koji Yamada; Hirohisa Omura πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 835 KB

Egg yolk lipoprotein promoted growth of a wide variety of mammalian cell lines, including plasma-cytomas and epithelial cell lines, in serum-free medium. The lipoprotein was active for cell growth when used with insulin, transferrin, ethanolamine and selenite. The most active lipoprotein fraction (Y

Serial cultivation of suspended BHK 21/1
Serial cultivation of suspended BHK 21/13 cells in serum-reduced and serum-free medium supplemented with various membrane protective agents
✍ S. Ismet GΓΌrhan; NilgΓΌn Γ–zdural πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 284 KB

Suspended BHK 21/13 cells were cultivated in 6M medium supplemented with PVP, bovine serum, LAH, YE, choline chloride and inositol at several concentrations. The maximum working capacity of the bioreactors was 400 ml and the experiments were run for 40 days. The growth promoting effects of each subs