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Growth of H-35 rat hepatoma cells in unsupplemented serum-free media: Effect of transferrin, insulin and cell density

โœ Scribed by Lawrence E. Shapiro; Neil Wagner


Book ID
112801131
Publisher
Society for In Vitro Biology
Year
1988
Tongue
English
Weight
419 KB
Volume
24
Category
Article
ISSN
1475-2689

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## Abstract HTC cells incubated in the absence of serum for more than 14 hours have very low levels of ornithine decarboxylase, the first enzyme on the pathway of polyamine synthesis. Readdition of serum causes an increase in the activity of ODC, reaching a maximum on average 17 times above the bas