Growth of Mouse Vaginal Epithelial Cells in Culture: Effect of Sera and Supplemented Serum-Free Media
โ Scribed by Taisen Iguchi, Francis-Dean A. Uchima and Howard A. Bern
- Book ID
- 125620532
- Publisher
- Society for In Vitro Biology
- Year
- 1987
- Tongue
- English
- Weight
- 283 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1475-2689
- DOI
- 10.2307/4296107
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The effect of lithium on the growth of mammary epithelial cells from adult virgin and midpregnant BALBlc or BALBlcfC3H mice was tested in a serumfree collagen gel culture system. The serum-free medium consisted of a 1:l mixture of Ham's F12 and Dulbecco's Modified Eagle's medium supplemented with in
Madin Darby canine kidney cells can grow in synthetic medium supplemented with 5 factors ~ insulin. transferrin, prostaglandin E , , hydrocortisone and triiodothyronine ~-as a seruni substitute. These 5 factors permit growth for one month in the absence of serum, and a growth rate equivalent t o tha