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Effect of growth conditions on the activity of ornithine decarboxylase in cultured hepatoma cells. II. Effect of serum and insulin

✍ Scribed by Brigid L. M. Hogan; Ann McIlhinney; Susan Murden

Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
386 KB
Volume
83
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

HTC cells incubated in the absence of serum for more than 14 hours have very low levels of ornithine decarboxylase, the first enzyme on the pathway of polyamine synthesis. Readdition of serum causes an increase in the activity of ODC, reaching a maximum on average 17 times above the basal level after five hours. This increase is due in part to a decrease in the apparent rate of degradation of ODC, and also to a stimulation of its synthesis. Within the first two hours the serum induction of ODC is resistant to Actinomycin D. Insulin at 5 ΞΌm/ml alsocauses an increase in ODC activity but only after a delay of two hours, in contrast to its more rapid stimulation of tyrosine transaminase activity.

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