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Effect of sodium arsentie on the induction and turnover of ornithine decarboxylase activity in erythroleukemia cells

✍ Scribed by Flavio Flamigni; Sandra Marmiroli; Claudio M. Caldarera; Carlo Guarnieri

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 347 KB
Volume: 7
Category: Article
ISSN: 0263-6484
DOI: 10.1002/cbf.290070310

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✦ Synopsis

Sodium arsenite proved effective in preventing the induction of ornithine decarboxylase (ODC) activity elicited by dilution of Friend erythroleukemia cells in fresh medium. A 50 per cent inhibition was produced at approximately 1 p~ arsenite and complete inhibition was obtained at concentrations above 1 0 ~~. However, addition of arsenite 5 h after cell dilution, i.e. when ODC was already induced, appeared to stabilize the enzyme. The half-life of ODC activity, measured after cycloheximide treatment, increased almost six-fold after addition of sodium arsenite. Agents known to provoke oxidative alteration of the thiol-redox status in cells, also caused a similar effect on the induction and stability of ODC.

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