## Abstract A human lung tumour cell line has been grown in serumβfree medium for 12 months and during this period cells were subcultured every 4 days. The culture medium contained an angiogenesis factor which has been purified and shown to be similar to that isolated from other sources including s
β¦ LIBER β¦
Growth of a human mammary tumour cell line in a serum-free medium
β Scribed by Barnes, David; Sato, Gordon
- Book ID
- 109716276
- Publisher
- Nature Publishing Group
- Year
- 1979
- Tongue
- English
- Weight
- 320 KB
- Volume
- 281
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/281388a0
No coin nor oath required. For personal study only.
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## Abstract The hormones which support growth, in vitro, of normal, neonatal human foreskin fibroblasts were determined. Wheresas thrombin and hydrocortisone were major growth stimulants, plateletβderived growth factor was not. Human foreskin fibroblasts grew in a serumβfree, biochemically defined