Differential requirements of two insect cell lines for growth in serum-free medium
β Scribed by J. L. Vaughn; F. Fan
- Book ID
- 107427487
- Publisher
- Society for In Vitro Biology
- Year
- 1997
- Tongue
- English
- Weight
- 411 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1475-2689
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π SIMILAR VOLUMES
The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum-free culture conditions. Sf9 cells in SF90011 medium apparently utilized sucrose as a primary nutrient both before and after virus infection, yielding small amounts of lactate and ammonia. Tn5 cells in Excell 401 medi
The lipid emulsion components of a serum-free insect cell medium were varied and evaluated for effects on cell growth and recombinant protein expression. The growth of High-Five(TM) cells was significantly affected by polyol Pluronic F-68 and Tween-80, but not by lipids. Pluronic was essential for c