Sulfated polyanions can be used to rapidly in-hancement of recombinant protein production (Dee, duce and maintain single-cell suspensions of BTIet al. 1997b). The enhancement may be a combined TN5B1-4 insect cells, a cell line which clumps in suspenresult of improved nutrient and oxygen transport, i
Low-Cost Serum-Free Medium for the BTI-Tn5B1–4 Insect Cell Line
✍ Scribed by Michael S. Donaldson; Michael L. Shuler
- Book ID
- 109388129
- Publisher
- American Institute of Chemical Engineers
- Year
- 1998
- Tongue
- English
- Weight
- 214 KB
- Volume
- 14
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Nutrient utilization and byproduct accumulation were monitored in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 (High Five™) cell lines during growth and following viral infection in suspension cultures in order to develop a better understanding of cell metabolism and to acquire inform
## Sulfated polyanions have been successfully significant advantage of this system is the high expresused to rapidly obtain and maintain stable single-cell sussion levels which can be achieved much more quickly pension of BTI-TN5B1-4 cells, a cell line which has a high than mammalian cell lines wh
Glycosylation is both cell line and protein dependent. Culture conditions can also influence the profile of glycoforms produced. To examine this possibility in the insect cell/baculovirus system, structures of N-linked oligosaccharides attached to SEAP (human secreted alkaline phosphatase), expresse