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Growth and G1 arrest of sarcoma virus transformed cells in serum free media

✍ Scribed by Merwin Moskowitz; David K. S. Cheng

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
849 KB
Volume
100
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Rous‐sarcoma transformed BHK cells can be continuously cultured in a medium containing Eagle's Minimal Essential Medium, iron and biotin. The rate of cell multiplication increased when serine, or serine plus other non‐essential amino‐acids were added to the medium. With biotin deleted from the medium there is a reduction in DNA synthesis and most cells are blocked in G1.

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