Grazing incidence surface-induced dissociation of protonated peptides generated by matrix-assisted laser desorption/ionization

✍ Scribed by Andreas Wieghaus; Lothar Schmidt; Anna M. Popova; Viatcheslav V. Komarov; Hartmut Jungclȧs

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 135 KB
• Volume: 14
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/1097-0231(20000930)14:18<1654::aid-rcm76>3.0.co;2-r

✦ Synopsis

The grazing incidence surface-induced dissociation (GI-SID) of various protonated peptides with typical kinetic energies of 350 eV was investigated. Peptide ions were generated by matrix-assisted laser desorption/ ionization (MALDI) using delayed extraction. The collision target surfaces used were aluminum and a liquid film of perfluorinated hydrocarbons. All peptides studied in these experiments showed enhanced fragment ion yields at grazing incidence (GI-SID effect) as observed in our former experiments with other precursor ion types. In general the GI-SID spectra exhibit N-terminal a 1 -type fragment ions, immonium ions and side-chain fragment ions in the low mass-to-charge region. Fragment ion series of the peptide backbone were not observed, which are typical and abundant in the spectra of established fragmentation techniques like collision-induced dissociation, MALDI post-source decay or surface-induced dissociation at steeper angles. The potential of the GI-SID process to yield useful information for primary structure determination of peptides is indicated by the observed differences in the GI-SID spectra of the isomeric dipeptides LR and IR.