Neutral and acidic oligosaccharides from human milk were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). These experiments require suitable matrices; their selection and particularly their preparation protocols must be optimized. Important criteria are sensitivi
Sequencing of oligosaccharides by collision-induced dissociation matrix-assisted laser desorption/ionization mass spectrometry
β Scribed by Emanuela Spina; Rosaria Cozzolino; Elizabeth Ryan; Domenico Garozzo
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 167 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1076-5174
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Matrix-assisted laser desorption/ionization combined with time-of-Γight mass spectrometry (MALDI/TOF-MS) was used for the analysis of low molecular mass compounds. Three classes of molecules were studied : organic acids, salts of oxyanions and amine-based chelating compounds. Mass spectra from sampl
Quantitative aspects of oligonucleotide analysis by matrix-assisted laser desorption/iotion (MALDI) mass spectrometry remain largely unexplored relative to the efforts that have been devoted to quantitative peptide and protein analysis. The successful quantitation of these other biopolymers coupled
Several polyoxymethylene (POM) model compounds with relatively low molecular weights were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using dithranol matrix, 1,1,1,3,3,3-hexafluoro-2-propanol solvent for matrix/analyte solution, and sodium iodide as cati
Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing