Partial 18 O-labeling of peptides has been applied to post-source decay experiments in a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The ions which originate from the carboxyl terminus of a peptide partially retain 18 O atoms which have readily been incorporated int
Advantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: sequencing of novel peptides from wasp venom
β Scribed by Miki Hisada; Katsuhiro Konno; Yasuhiro Itagaki; Hideo Naoki; Terumi Nakajima
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 161 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0951-4198
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## Abstract Highβsensitivity, rapid identification of proteins in proteomic studies normally uses a combination of oneβ or twoβdimensional electrophoresis together with mass spectrometry. The simplicity and sensitivity of matrixβassisted laser desorption/ionization timeβofβflight mass spectrometry