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Discrimination of isomeric oligosaccharides and sequencing of unknowns by post source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Scribed by Domenico Garozzo; Valeria Nasello; Emanuela Spina; Luisa Sturiale

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 175 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199709)11:14<1561::aid-rcm45>3.0.co;2-x

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✦ Synopsis

The PSD spectra of some isomeric oligosaccharides are investigated in order to study the fragmentation mechanisms. Results show that intense peaks are formed from one or more glycosidic linkage cleavages, while ring fragmentation produces very weak signals. Two analytical applications of post source decay (PSD) to the structural analysis of oligosaccharides are also illustrated: the discrimination of isomeric structures and the identification of a real unknown by combining PSD data with the linkage analysis results.

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