𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Gradient, high-resolution, magic-angle spinning nuclear magnetic resonance spectroscopy of human adipocyte tissue

✍ Scribed by Kevin K. Millis; Werner E. Maas; David G. Cory; Samuel Singer

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
545 KB
Volume
38
Category
Article
ISSN
0740-3194

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The recently developed technique of gradient, high‐resolution magic‐angle spinning NMR (g‐hr‐MAS‐NMR) spectroscopy was applied to the study of ex vivo human lipoma and liposarcoma tissue. Compared with conventional ^1^H‐NMR, the g‐hr‐MAS method yielded a large improvement in spectral resolution and permitted the detection of metabolite resonance's in a well‐differentiated liposarcoma that was not observed in spectra of similar samples obtained using nonspinning NMR methods. These findings suggest that g‐hr‐MAS‐NMR spectroscopy provides a key improvement in spectral quality for ex vivo lipoma and liposarcoma tissue thereby permitting a more precise determination of tissue metabolite composition than conventional nonspinning NMR methods. Key words: magic‐angle spinning; nuclear magnetic resonance; magnetic field gradients; sarcoma.

📜 SIMILAR VOLUMES

Gradient, high-resolution, magic angle s
Gradient, high-resolution, magic angle spinning 1H nuclear magnetic resonance spectroscopy of intact cells
✍ Patrick Weybright; Kevin Millis; Natalee Campbell; David G. Cory; Samuel Singer 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 917 KB

## Abstract The application of new gradient, high‐resolution, magic angle spinning (MAS) ^1^H nuclear magnetic resonance (NMR) spectroscopy to the study of intact undifferentiated and differentiated NIH 3T3 F442A cells demonstrated improved spectral resolution and sensitivity compared with static s

High-resolution nuclear magnetic resonan
High-resolution nuclear magnetic resonance spectroscopy of biological tissues using projected magic angle spinning
✍ Rachel W. Martin; Rebecca C. Jachmann; Dimitris Sakellariou; Ulla Gro Nielsen; A 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 569 KB

## Abstract High‐resolution NMR spectra of materials subject to anisotropic broadening are usually obtained by rotating the sample about the magic angle, which is 54.7° to the static magnetic field. In projected magic angle spinning (p‐MAS), the sample is spun about two angles, neither of which is

Evaluating Human Breast Ductal Carcinoma
Evaluating Human Breast Ductal Carcinomas with High-Resolution Magic-Angle Spinning Proton Magnetic Resonance Spectroscopy
✍ Leo Ling Cheng; I-Wen Chang; Barbara L. Smith; R.Gilberto Gonzalez 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 159 KB

We report the results of a study of human breast ductal carcinomas, conducted by using high resolution magic angle spinning proton magnetic resonance spectroscopy (HRMAS 1HMRS). This recently developed spectroscopic technique can measure tissue metabolism from intact pathological specimens and ident

Biochemical correlates of thiazolidinedi
Biochemical correlates of thiazolidinedione-induced adipocyte differentiation by high-resolution magic angle spinning NMR spectroscopy
✍ Jin-Hong Chen; Brian M. Enloe; Patrick Weybright; Natalee Campbell; David Dorfma 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 689 KB

## Abstract Thiazolidinediones, a class of synthetic ligands to the peroxisome proliferator‐activated receptor‐γ, induce terminal adipocyte differentiation of 3T3 F442A cells, and have already been used as alternative therapeutic agents for the treatment of liposarcoma in clinical trials. The bioch

High-resolution magic-angle spinning 13C
High-resolution magic-angle spinning 13C spectroscopy of brain tissue at natural abundance
✍ YongXia Yang; Lei Chen; HongChang Gao; DanLin Zeng; Yong Yue; MaiLi Liu; Hao Lei 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 178 KB 👁 1 views

## Abstract High‐resolution magic‐angle spinning (MAS) ^1^H and ^13^C magnetic resonance spectroscopy (MRS) has recently been applied to study the metabolism in intact biological tissue samples. Because of the low natural abundance and the low gyromagnetic ratio of the ^13^C nuclei, signal enhancem