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Gradient, high-resolution, magic angle spinning 1H nuclear magnetic resonance spectroscopy of intact cells

✍ Scribed by Patrick Weybright; Kevin Millis; Natalee Campbell; David G. Cory; Samuel Singer

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
917 KB
Volume
39
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The application of new gradient, high‐resolution, magic angle spinning (MAS) ^1^H nuclear magnetic resonance (NMR) spectroscopy to the study of intact undifferentiated and differentiated NIH 3T3 F442A cells demonstrated improved spectral resolution and sensitivity compared with static studies. MAS of cells permits the detection and quantitation of many cellular metabolites that are not clearly resolved in nonspinning measurements and provides an improved visibility of phospholipids. Gradient, MAS enables the use of diffusion weighting for compartment assignment and the determination of mobility for many metabolites which are incompletely resolved using static techniques. The smaller, undifferentiated preadipocytes show no microscopic evidence of cell lysis after 2 h of MAS at 3.5 kHz and 82% of these cells remain viable by trypan blue exclusion. In contrast, 15‐19% of the larger, lipid‐laden differentiated adipocytes were found to suffer some degree of cell lysis with MAS. This new method is an attractive alternative to either nonspinning perfusion or extraction techniques for NMR studies of cells.

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