Abstract
High‐resolution magic‐angle spinning (MAS) ^1^H and ^13^C magnetic resonance spectroscopy (MRS) has recently been applied to study the metabolism in intact biological tissue samples. Because of the low natural abundance and the low gyromagnetic ratio of the ^13^C nuclei, signal enhancement techniques such as cross‐polarization (CP) and distortionless enhancement by polarization transfer (DEPT) are often employed in MAS ^13^C MRS to improve the detection sensitivity. In this study, several sensitivity enhancement techniques commonly used in liquid‐ and solid‐state NMR, including CP, DEPT and nuclear Overhauser enhancement (NOE), were combined with MAS to acquire high‐resolution ^13^C spectra on intact rat brain tissue at natural abundance, and were compared for their performances. The results showed that different signal enhancement techniques are sensitive to different classes of molecules/metabolites, depending on their molecular weights and mobility. DEPT was found to enhance the signals of low–molecular weight metabolites exclusively, while the signals of lipids, which often are associated with membranes and have relatively lower mobility, were highly sensitive to CP enhancement. Copyright © 2006 John Wiley & Sons, Ltd.