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Going green in Cryptococcus neoformans: The recycling of a selectable drug marker

โœ Scribed by Raunak D. Patel; Jennifer K. Lodge; Lorina G. Baker


Book ID
116482061
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
354 KB
Volume
47
Category
Article
ISSN
1087-1845

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A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic