A novel episomal shuttle vector for transformation of Cryptococcus neoformans with the ccdB gene as a positive selection marker in bacteria

Characterization of the L41 gene in Cryp

Characterization of the L41 gene in Cryptococcus neoformans: its application as a selectable transformation marker for cycloheximide resistance

✍ A. Varma; K. J. Kwon-Chung 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 174 KB 👁 2 views

A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic